منابع مشابه
Mixed Agglutination with Tissue Sections
The mixed agglutination procedure was applied to tissue sections. Microtome sections of bovine tissue were placed on coverglasses, fixed by acetone or formalin, and incubated with various dilutions of rabbit antisera. The binding of antibodies to the tissue sections was detected by the addition of an indicator system composed of sheep erythrocytes sensitized by subagglutinating doses of the cor...
متن کاملارزیابی بالینی نتایج آزمایش مار(MAR– Mixed Agglutination Reaction) و آزمایش تات (TAT – Tray Agglutination Test) در مردان نابارور
ABSTRACT: Of the antisperm immunoglobulins ,only antisperm surface IgA an IgGs are significant clinically because many fertile men have antisperm antibodies present in their sera. Since the necessity of one form of sperm surface antisperm test(specific)is felt in our country,and Immunobead is not popular here for its high cost,Mixed Antiglobulin Reaction test was studied in a population of inf...
متن کاملPreparation of Tissue Sections
A simple reproducible protocol for detecting multiple copy human genes and viral DNA in routine formalin fixed paraffin embedded tonsil and brain, by in situ hybridisation with biotinylated probes, is described. The protocol consists of digestion of formalin fixed paraffin sections, with 0 4% pepsin in 0 01 M hydrochloric acid for one hour at 37°C, followed by hybridisation with biotinylated pr...
متن کاملThe effect of antisperm antibodies measured by direct mixed agglutination reaction on in vitro fertilization rate
Background: Although in vitro fertilization (IVF) has been found to bypass the inhibitory effect of high levels of antisperm antibodies on sperm motility, fertilization rates are reduced in approximately 40% of the attempts. In this study, we investigated the relationship between antisperm antibodies measured by the direct mixed agglutination reaction (MAR) and the fertilization rate in inferti...
متن کاملCharacterization of hyaluronic acid on tissue sections with hyaluronectin.
An affinity immunological procedure for hyaluronic acid detection on tissue sections is described. This new, sensitive, and specific technique is based on the high affinity of hyaluronectin for hyaluronic acid, utilizing anti-hyaluronectin-hyaluronectin immune complexes. Elimination of binding when the reagent was supplemented with hyaluronic acid or when Streptomyces hyaluronidase-digested tis...
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ژورنال
عنوان ژورنال: Journal of Experimental Medicine
سال: 1964
ISSN: 1540-9538,0022-1007
DOI: 10.1084/jem.119.2.265